抗体 Anti-beta I Tubulin Antibody (8S183), Loading Control|TMAB-03117|TargetMol

中文名称：

抗体 Anti-beta I Tubulin Antibody (8S183), Loading Control

英文名称：

Anti-beta I Tubulin Antibody (8S183), Loading Control

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Antibody

种属：

Human

应用：

WB,IHC-P,IHC-Fr,IF,ICC/IF,FCM

反应性：

Human,Mouse,Rat

宿主：

Rabbit

靶点：

TUBB1

克隆性：

Monoclonal

免疫原：

KLH conjugated synthetic peptide: human beta I Tubulin

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated (TMAB-03117) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated (TMAB-03117) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated (TMAB-03117) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated (TMAB-03117) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated (TMAB-03117) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated (TMAB-03117) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated (TMAB-03117) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 8. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (beta I Tubulin) monoclonal Antibody, unconjugated (TMAB-03117) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, FITC) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control. 9. 25 ug total protein per lane of various lysates (see on figure) probed with beta I Tubulin monoclonal antibody, unconjugated (TMAB-03117) at 1:50000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

UniProt ID：

Q9H4B7

克隆号：

8S183

浓度：

1mg/ml

基因名称：

TUBB1

货号：

T014|TMAB-03117