抗体 Anti-Caldesmon Antibody (3N11)|TMAB-03571|TargetMol

中文名称：

抗体 Anti-Caldesmon Antibody (3N11)

英文名称：

Anti-Caldesmon Antibody (3N11)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Antibody

应用：

WB,IHC-P,IHC-Fr,IF

反应性：

Human,Mouse,Rat

宿主：

Mouse

靶点：

CALD1

克隆性：

Monoclonal

免疫原：

KLH conjugated synthetic peptide: human Caldesmon

亚型：

IgG2b/Kappa

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CALD1) Monoclonal Antibody, Unconjugated (TMAB-03571) at 1: 100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CALD1) Monoclonal Antibody, Unconjugated (TMAB-03571) at 1: 100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CALD1) Monoclonal Antibody, Unconjugated (TMAB-03571) at 1: 100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CALD1) Monoclonal Antibody, Unconjugated (TMAB-03571) at 1: 100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CALD1) Monoclonal Antibody, Unconjugated (TMAB-03571) at 1: 100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CALD1) Monoclonal Antibody, Unconjugated (TMAB-03571) at 1: 100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CALD1) Monoclonal Antibody, Unconjugated (TMAB-03571) at 1: 100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded (mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CALD1) Monoclonal Antibody, Unconjugated (TMAB-03571) at 1: 100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded (human uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CALD1) Monoclonal Antibody, Unconjugated (TMAB-03571) at 1: 100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 10. 25 ug total protein per lane of various lysates (see on figure) probed with Caldesmon monoclonal antibody, unconjugated (TMAB-03571) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

UniProt ID：

Q05682

克隆号：

3N11

浓度：

1mg/ml

基因名称：

CALD1

货号：

T014|TMAB-03571