抗体 Anti-14-3-3 Polyclonal Antibody|TMAB-02084|TargetMol

中文名称：

抗体 Anti-14-3-3 Polyclonal Antibody

英文名称：

Anti-14-3-3 Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

WB,IHC-P,IHC-Fr,IF,FCM

反应性：

Human,Mouse,Rat (predicted:Sheep,Fruit Fly,Yeast)

宿主：

Rabbit

靶点：

YWHAZ

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide derived from human pan 14-3-3

亚型：

IgG

验证方法：

1. Blank control (blue line): HL60 (fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% P Dilution: 1 μg /test. 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (14-3-3) Polyclonal Antibody, Unconjugated (TMAB-02084) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Blank control: A431. Primary Antibody (green line): Rabbit Anti-14-3-3 Alpha+Beta+Gamma+Delta+Epsilon antibody (TMAB-02084) Dilution: 1 μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg /test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. 4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (14-3-3) Polyclonal Antibody, Unconjugated (TMAB-02084) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

UniProt ID：

P63104

浓度：

1mg/ml

基因名称：

YWHAZ

货号：

T014|TMAB-02084