抗体 Anti-ABCE1 Antibody (3T571)|TMAB-02157|TargetMol

中文名称：

抗体 Anti-ABCE1 Antibody (3T571)

英文名称：

Anti-ABCE1 Antibody (3T571)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Antibody

应用：

WB,IHC-P,IHC-F,IF,FCM,IP

反应性：

Human,Mouse,Rat

宿主：

Rabbit

靶点：

ABCE1

克隆性：

Monoclonal

免疫原：

A synthesized peptide: human ABCE1

亚型：

IgG/Kappa

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ABCE1 Monoclonal Antibody, Unconjugated (TMAB-02157) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ABCE1 Monoclonal Antibody, Unconjugated (TMAB-02157) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ABCE1 Monoclonal Antibody, Unconjugated (TMAB-02157) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. 25 μg total protein per lane of various lysates (see on figure) probed with ABCE1 monoclonal antibody, unconjugated (TMAB-02157) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 5. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ABCE1 antibody (TMAB-02157, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

UniProt ID：

P61221

克隆号：

3T571

浓度：

1mg/ml

基因名称：

ABCE1

货号：

T014|TMAB-02157