等电压依赖性阴离子通道（内参）重组兔单抗|TMAB-01943|TargetMol

中文名称：

等电压依赖性阴离子通道（内参）重组兔单抗

英文名称：

VDAC1 Mitochondrial Loading Control Antibody (5D173)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Antibody

种属：

Human

应用：

ICC/IF,IF,IHC-Fr,IHC-P,WB

反应性：

Human,Mouse,Rat

宿主：

Rabbit

靶点：

VDAC1

克隆性：

Monoclonal

免疫原：

A synthesized peptide: human VDAC1

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (VDAC1) Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (VDAC1) Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 13. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 14. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 15. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated (TMAB-01943) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 16. 25 μg total protein per Lane of various lysates probed with VDAC1 monoclonal antibody, unconjugated (TMAB-01943) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 17. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (VDAC1) monoclonal Antibody, unconjugated (TMAB-01943) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green ) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

UniProt ID：

P21796

克隆号：

5D173

浓度：

1 mg/mL

基因名称：

VDAC1

货号：

T014|TMAB-01943