人Claudin-3全长蛋白(VLP)

Human Claudin-3 Full Length Protein (VLP)

分子别名（Synonym）

C7orf1, CPE-R2, CPETR2, HRVP1, RVP1

表达区间及表达系统（Source）

Human Claudin-3 Full Length Protein (VLP) (CL3-H52P3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Val 220 (Accession # O15551).

Predicted N-terminus: Met 1

蛋白结构（Molecular Characterization）

Virus-like particles(VLPs) are formed by self-assembly of capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.

The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SEC-HPLC.

制剂（Formulation）

The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product MUST be stored at -70°C or lower upon receipt;

-70°C for 12 months under sterile conditions.

背景介绍

紧密连接是上皮或内皮细胞片中细胞间黏连的一种方式，它们在细胞周围形成连续的封闭结构，作为物理屏障，阻止溶质和水自由通过细胞旁间隙。这些连接由面向细胞质的脂质双层外层中一系列连续的网络链组成，并在面向细胞外的脂质双层内层中形成互补的沟槽。该基因（无内含子）编码的蛋白是claudin家族成员，一种整合膜蛋白，也是紧密连接链的组成部分。该蛋白同时也是产气荚膜梭菌肠毒素的低亲和力受体，并且与大鼠中一种推测的凋亡相关蛋白具有氨基酸序列相似性。