CD8重组兔单抗|TMAB-00391|TargetMol

中文名称：

抗体 Anti-CD8 alpha Monoclonal Antibody (9O552)

英文名称：

Anti-CD8 alpha Monoclonal Antibody (9O552)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Antibody

种属：

Human

应用：

IF,IHC-Fr,IHC-P,WB

反应性：

Human

宿主：

Rabbit

靶点：

CD8A

克隆性：

Monoclonal

免疫原：

KLH conjugated synthetic peptide: human CD8

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. 25 μg total protein per Lane of various lysates probed with CD8 monoclonal antibody, unconjugated (TMAB-00391) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 4. Paraformaldehyde-fixed, paraffin embedded (human squamous cell lung cancer); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

UniProt ID：

P01732

克隆号：

9O552

基因名称：

CD8A

货号：

T014|TMAB-00391