细胞色素C抗体|TMAB-00516|TargetMol

中文名称：

细胞色素C抗体

英文名称：

Anti-CYCS Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

WB,IHC-P,IHC-Fr,IF

反应性：

Mouse,Rat,Human (predicted:Cow)

宿主：

Rabbit

靶点：

CYCS

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide: human Cytochrome C

亚型：

IgG

验证方法：

1. Blank control: HepG2 (blue). Primary Antibody: Rabbit Anti-Cytochrome C antibody (TMAB-00516,Green); Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at 37°C. Primary antibody (TMAB-00516, 1 μg/1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. 2. Tissue/cell: SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Cytochrome C) polyclonal Antibody, Unconjugated (TMAB-00516) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 3. Sample: Lane 1: Heart (Rat) Lysate at 40 μg Lane 2: Heart (Mouse) Lysate at 40 μg Primary: Anti-Cytochrome C (TMAB-00516) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 14.4 kDa Observed band size: 14.4 kDa

UniProt ID：

P99999

浓度：

1 mg/mL

基因名称：

CYCS

货号：

T014|TMAB-00516