丝裂原活化蛋白激酶1/2抗体|TMAB-00634|TargetMol

中文名称：

丝裂原活化蛋白激酶1/2抗体

英文名称：

Anti-ERK1/2 Polyclonal Antibody 2

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

FCM,ICC/IF,IF,IHC-Fr,IHC-P,WB

反应性：

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,Goat)

宿主：

Rabbit

靶点：

MAPK3

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide derived from human ERK1/2

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated (TMAB-00634) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated (TMAB-00634) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 3. Sample: Lane 1: A431 (Human) Cell Lysate at 30 μg Lane 2: Cerebrum (Mouse) Lysate at 40 μg Lane 3: Cerebrum (Rat) Lysate at 40 μg Lane 4: LympHnode (Mouse) Lysate at 40 μg Lane 5: LympHnode (Rat) Lysate at 40 μg Lane 6: SiHa (Human) Cell Lysate at 30 μg Lane 7: HUVEC (Human) Cell Lysate at 30 μg Primary: Anti-ERK1 + ERK2 (TMAB-00634) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44/42 kDa Observed band size: 38 kDa

UniProt ID：

P27361

浓度：

1 mg/mL

基因名称：

MAPK3

货号：

T014|TMAB-00634