I型胶原蛋白/胶原蛋白1/1型胶原蛋白/I型胶原a1抗体|TMAB-00455|TargetMol

中文名称：

I型胶原蛋白/胶原蛋白1/1型胶原蛋白/I型胶原a1抗体

英文名称：

Anti-Collagen I/COL1A1 Polyclonal Antibody 2

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

FCM,ICC/IF,IF,IHC-Fr,IHC-P,WB

反应性：

Human,Mouse,Rat

宿主：

Rabbit

靶点：

COL1A1

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide: human Collagen I

亚型：

IgG

验证方法：

1. 25 μg total protein per Lane of various lysates probed with Collagen I polyclonal antibody, unconjugated (TMAB-00455) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 2. U-118 MG (H) cells were treated with or without BFA, 25 μg total protein per Lane of cell lysates probed with Collagen I polyclonal antibody, unconjugated (TMAB-00455) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 13. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 14. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 15. Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 16. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 17. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 18. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 19. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Polyclonal Antibody, Unconjugated (TMAB-00455) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 20. 4% Paraformaldehyde-fixed U-87MG (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (Collagen I) polyclonal Antibody, unconjugated (TMAB-00455) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. 21. The U-87MG (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-Collagen I antibody (TMAB-00455,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test.Blank control (black): PBS. 22. The U2-OS (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-Collagen I antibody (TMAB-00455,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Blank control (black): PBS. 23. 4% Paraformaldehyde-fixed U2-OS (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (Collagen I) polyclonal Antibody, unconjugated (TMAB-00455) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

UniProt ID：

P02452

浓度：

1 mg/mL

基因名称：

COL1A1

货号：

T014|TMAB-00455