微管蛋白β tubulin/Tubulin β（内参）抗体|TMAB-00236|TargetMol

中文名称：

微管蛋白β tubulin/Tubulin β（内参）抗体

英文名称：

Beta-Tubulin Loading Control Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

FCM,IF,IHC-Fr,IHC-P,WB

反应性：

Human,Mouse,Rat

宿主：

Rabbit

靶点：

TUBB

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide: human tubulin Beta

亚型：

IgG

验证方法：

1. Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-Beta tubulin antibody (TMAB-00236) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 2. Sample: Lane 1,3,5: Mouse Cerebellum tissue lysates Lane 2,4,6: Rat Cerebellum tissue lysates Primary: Anti-Beta tubulin (TMAB-00236) at 1/10000~1/50000 dilution Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 48 kDa 3. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (TMAB-00236) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (TMAB-00236) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (TMAB-00236) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (TMAB-00236) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

UniProt ID：

P07437

浓度：

1 mg/mL

基因名称：

TUBB

货号：

T014|TMAB-00236