晚期糖基化终末产物抗体|TMAB-00075|TargetMol

中文名称：

晚期糖基化终末产物抗体

英文名称：

Anti-AGEs Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

应用：

FCM,IF,IHC-Fr,IHC-P,WB

反应性：

Human,Mouse,Rat

宿主：

Rabbit

靶点：

ALB

克隆性：

Polyclonal

免疫原：

AGEs

亚型：

IgG

验证方法：

1. Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-AGEs Polyclonal Antibody, Unconjugated (TMAB-00075) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-AGEs antibody (TMAB-00075) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 3. 25 μg total protein per Lane of various lysates probed with AGEs polyclonal antibody, unconjugated (TMAB-00075) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 4. 25 μg total protein per Lane of various lysates probed with AGEs polyclonal antibody, unconjugated (TMAB-00075) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

浓度：

1 mg/mL

基因名称：

ALB

货号：

T014|TMAB-00075