芳香烃受体抗体|TMAB-00081|TargetMol

中文名称：

芳香烃受体抗体

英文名称：

Anti-AHR Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

FCM,ICC/IF,IF,IHC-Fr,IHC-P

反应性：

Human,Rat (predicted:Mouse)

宿主：

Rabbit

靶点：

AHR

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide: human AHR

亚型：

IgG

验证方法：

1. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (AHR) polyclonal Antibody, Unconjugated (TMAB-00080) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-AHR Polyclonal Antibody, Unconjugated (TMAB-00080) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-AHR Polyclonal Antibody, Unconjugated (TMAB-00080) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Blank control: U937. Primary Antibody (green line): Rabbit Anti-AHR antibody (TMAB-00080) Dilution: 2 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

UniProt ID：

P35869

浓度：

1 mg/mL

基因名称：

AHR

货号：

T014|TMAB-00080