嗜铬粒素A抗体|TMAB-00424|TargetMol

中文名称：

嗜铬粒素A抗体

英文名称：

Anti-CHGA Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

ICC/IF,IF,IHC-Fr,IHC-P

反应性：

Human,Rat (predicted:Mouse)

宿主：

Rabbit

靶点：

CHGA

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide: human CHGA

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Chromogranin A) Polyclonal Antibody, Unconjugated (TMAB-00424) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Chromogranin A) Polyclonal Antibody, Unconjugated (TMAB-00424) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Chromogranin A) Polyclonal Antibody, Unconjugated (TMAB-00424) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Chromogranin A) polyclonal Antibody, Unconjugated (TMAB-00424) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

UniProt ID：

P10645

浓度：

1 mg/mL

基因名称：

CHGA

货号：

T014|TMAB-00424