钙/钙调素依赖蛋白激酶2α抗体|TMAB-00275|TargetMol

中文名称：

钙/钙调素依赖蛋白激酶2α抗体

英文名称：

Anti-CAMK2 alpha Polyclonal Antibody 2

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

ICC/IF,IF,IHC-Fr,IHC-P,WB

反应性：

Human,Mouse,Rat

宿主：

Rabbit

靶点：

CAMK2A

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide derived from human CaMK2 alpha

亚型：

IgG

验证方法：

1. Sample: Lane 1: Mouse Heart tissue lysates Lane 2: Rat Heart tissue lysates Primary: Anti-CaMK2 alpha (TMAB-00275) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 50 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMK2 alpha) Polyclonal Antibody, Unconjugated (TMAB-00275) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. 25 μg total protein per Lane of various lysates probed with CaMK2 alpha polyclonal antibody, unconjugated (TMAB-00275) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 4. 4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (CaMK2 alpha) polyclonal Antibody, unconjugated (TMAB-00275) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

UniProt ID：

Q9UQM7

浓度：

1 mg/mL

基因名称：

CAMK2A

货号：

T014|TMAB-00275