载脂蛋白1抗体|TMAB-00657|TargetMol

中文名称：

载脂蛋白1抗体

英文名称：

Anti-Fas/CD95 Polyclonal Antibody 2

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

FCM,WB

反应性：

Human,Mouse,Rat (predicted:Pig)

宿主：

Rabbit

靶点：

FAS

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide: human FAS/Apo-1/CD95

亚型：

IgG

验证方法：

1. Blank control: Raji. Primary Antibody (green line): Rabbit Anti-CD95/FAS antibody (TMAB-00657) Dilution: 2 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-AF488 Dilution: 0.5 μg/Test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 2. Blank control (blue): Mouse Kidney (fixed with 2% paraformaldehyde for 10 min at 37°C). Primary Antibody: Rabbit Anti-CD95/FAS antibody (TMAB-00657,Green); Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. 3. Sample: Lane 1: Thymus (Mouse) Lysate at 40 μg Lane 2: Thymus (Rat) Lysate at 40 μg Lane 3: HepG2 (Human) Cell Lysate at 30 μg Lane 4: Raji (Human) Cell Lysate at 30 μg Lane 5: HT1080 (Human) Cell Lysate at 30 μg Lane 6: A431 (Human) Cell Lysate at 30 μg Primary: Anti-CD95/FAS (TMAB-00657) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45/52 kDa Observed band size: 52 kDa 4. Sample: serum (Rat) at 40 μg plasma (Rat) at 40 μg Primary: Anti-CD95 (TMAB-00657) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 42 kDa

UniProt ID：

P25445

浓度：

1 mg/mL

基因名称：

FAS

货号：

T014|TMAB-00657