内皮素-1抗体|TMAB-00611|TargetMol

中文名称：

抗体 Anti-Endothelin-1/EDN1 Polyclonal Antibody

英文名称：

Anti-Endothelin-1/EDN1 Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

WB,IHC-P,ICC/IF,ELISA

反应性：

Human,Mouse,Rat

宿主：

Rabbit

偶联物：

Unconjugated

靶点：

EDN1

克隆性：

Polyclonal

免疫原：

The antiserum was produced against synthesized peptide derived from the Internal region of human EDN1.

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Big endothelin-1) Polyclonal Antibody, Unconjugated (TMAB-00611) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Big endothelin-1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Big endothelin-1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Sample: Lane 1: Testis (Mouse) Lysate at 40 μg Lane 2: Testis (Rat) Lysate at 40 μg Primary: Anti-Big endothelin-1 (TMAB-00611) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 26 kDa

UniProt ID：

P05305

浓度：

1 mg/mL

基因名称：

EDN1

货号：

T014|TMAB-00611