纤维状肌动蛋白抗体|TMAB-00217|TargetMol

中文名称：

纤维状肌动蛋白抗体

英文名称：

Beta-Actin Loading Control Polyclonal Antibody 2

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

FCM,IF,IHC-Fr,IHC-P,WB

反应性：

Human,Mouse,Rat (predicted:Pig,Cow,Sheep)

宿主：

Rabbit

靶点：

ACTB

克隆性：

Polyclonal

免疫原：

Synthetic MAP peptide derived from human beta-Actin

亚型：

IgG

验证方法：

1. Sample: Lane1: Hela (Human) Whole Cell Lysate at 30 μg Lane2: Jurkat (Human) Whole Cell Lysate at 30 μg Lane3: 293T (Human) Whole Cell Lysate at 30 μg Lane4: Hepg2 (Human) Whole Cell Lysate at 30 μg Lane5: A431 (Human) Whole Cell Lysate at 30 μg Lane6: A549 (Human) Whole Cell Lysate at 30 μg Lane7: H9C2 (Rat) Whole Cell Lysate at 30 μg Lane8: Testis (Mouse) Tissue Lysate at 30 μg Primary: Anti-F-Actin (TMAB-00217) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa 2. Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-F-Actin antibody (TMAB-00217) Dilution: 3 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1 μg/test. Protocol The cells were fixed with 80% methanol (5 min at-20°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. 3. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (TMAB-00217) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (TMAB-00217) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

UniProt ID：

P60709

浓度：

1 mg/mL

基因名称：

ACTB

货号：

T014|TMAB-00217