All-In-One 5X RT MasterMix with gDNA Removal

The All-In-One 5X RT MasterMix with gDNA Removal offers a convenient, ready-to-use solution for first-strand cDNA synthesis with genomic DNA removal, all in a single tube. This powerful formulation addresses the common challenge of genomic DNA contamination, ensuring accurate RNA detection without compromising reverse transcription or cDNA synthesis.

The optimized MasterMix includes abm’s proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a carefully balanced mix of Oligo(dT)s and Random Primers. The OneScript® Hot Reverse Transcriptase features genetic modifications that abolish RNase H activity to achieve thermal stability, allowing the RNA template to remain intact during reverse transcription for enhanced cDNA yield and integrity. By eliminating the need for multiple separate reagents, this all-in-one mix offers a straightforward, reliable, and reproducible workflow that consistently delivers high-quality cDNA, even from challenging or low-quality RNA samples—ideal for a wide range of downstream molecular biology applications.

Product Features:

• Easy one-step setup reduces pipetting and sample handling

• Removes contaminating gDNA with temperature-sensitive DNase

• High cDNA yields from difficult samples with OneScript® Hot Reverse Transcriptase (Cat. No. G593)

• Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G138)

• Also includes dNTPs, Oligo (dT)s, and random primers