磷酸化丝裂原活化蛋白激酶1/2抗体 新品

磷酸化丝裂原活化蛋白激酶1/2抗体

原理特点

磷酸化ERK1/2是MAPK通路核心活化标志物，调控细胞增殖、分化、迁移。该抗体广谱识别两种亚型磷酸化ERK，整体检测ERK通路活化水平；阻断激酶活性可抑制细胞异常增殖与迁移。
英文名称:phospho-ERK1 + 2 (Thr183/Tyr185) Rabbit pAb
中文名称:磷酸化丝裂原活化蛋白激酶1/2抗体

Not yet tested in other applications.

Optimal working dilutions must be determined by the end user.

英文别名:ERK1+ERK2(phospho Thr183/Tyr185); phospho-ERK1/MAPK-1/2(Thr183/Tyr185); ERK 1; ERK 2; ERK-2; ERK1; ERK2; ERT1; ERT2; Extracellular signal regulated kinase 1; Extracellular signal regulated kinase 1; Extracellular signal regulated kinase 2; Extracellular signal regulated kinase 2; Extracellular signal-regulated kinase 2; HS44KDAP; HUMKER1A; Insulin stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK1; MAPK2; MGC20180; Microtubule associated protein 2 kinase; Mitogen activated protein kinase 1; Mitogen activated protein kinase 1; Mitogen activated protein kinase 2; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; MK01_MOUSE; p38; p40; p41; p41mapk; p42 MAPK; p42-MAPK; p42MAPK; p42MAPK; p44 ERK1; p44 MAPK; p44ERK1; p44ERK1; p44MAPK; p44MAPK; PRKM 1; PRKM 1; PRKM 2; PRKM 2; PRKM1; PRKM2; Protein kinase mitogen activated 1; Protein kinase mitogen activated 1; Protein kinase mitogen activated 2; Protein kinase mitogen activated 2; Protein tyrosine kinas.

交叉反应:Human

抗体来源:Rabbit

免疫原:KLH conjugated Synthesised phosphopeptide derived from mouse ERK1 around the phosphorylation site of Thr183/Tyr185

亚型:IgG

性状:Liquid

纯化方法:affinity purified by Protein A

克隆类型:Polyclonal

理论分子量：42/44 kDa

浓度：1mg/ml

储存液：0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件：Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
靶点背景

靶点背景：ERK1（MAPK1）、ERK2（MAPK2）受 Ras-Raf-MEK 级联双位点磷酸化激活，调控细胞增殖、分化、迁移。

抗体原理：靶向 ERK1/2 共同保守磷酸化基序肽段，可同时识别磷酸化 ERK1 与 ERK2 两条条带，不结合静息总 ERK。

产品特点：科研经典通路抗体，细胞、组织样本通用，肿瘤、纤维化、创伤修复模型标配检测试剂。
抗体的鉴定

1）抗体的效价鉴定：鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。

3）抗体的亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。

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