c-fos抗体|TMAB-00699|TargetMol

中文名称：

c-fos抗体

英文名称：

Anti-FOS Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

ICC/IF,IF,IHC-Fr,IHC-P,WB

反应性：

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit)

宿主：

Rabbit

靶点：

FOS

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide: human c-fos

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (TMAB-00698) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (TMAB-00698) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Tissue/cell: A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (TMAB-00698) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 4. Tissue/cell: MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (TMAB-00698) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 5. Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (TMAB-00698) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (TMAB-00698) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. MCF-7 Cell (Human) Lysate at 30 μg Embryo (Mouse) Lysate at 40 μg RAW246.7 (Mouse) lysate at 30 μg NIH/3T3 (Mouse) lysate at 30 μg Cerebrum (Mouse) Lysate at 40 μg Cerebrum (Rat) Lysate at 40 μg Primary: Anti-c-fos (TMAB-00698) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kDa Observed band size: 55 kDa

UniProt ID：

P01100

浓度：

1 mg/mL

基因名称：

FOS

货号：

T014|TMAB-00698