肝细胞核因子4α抗体|TMAB-00877|TargetMol

中文名称：

肝细胞核因子4α抗体

英文名称：

Anti-HNF4A Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

WB,IHC-P,IHC-Fr,IF,IP,ChIP

反应性：

Human,Mouse,Rat

宿主：

Rabbit

靶点：

HNF4A

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide: human HNF4 alpha

亚型：

IgG

验证方法：

1. 25 μg total protein per lane of various lysates (see on figure) probed with HNF4A polyclonal antibody, unconjugated (TMAB-00877) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HNF4A Polyclonal Antibody, Unconjugated (TMAB-00877) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HNF4A Polyclonal Antibody, Unconjugated (TMAB-00877) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HNF4A Polyclonal Antibody, Unconjugated (TMAB-00877) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HNF4A Polyclonal Antibody, Unconjugated (TMAB-00877) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HNF4A Polyclonal Antibody, Unconjugated (TMAB-00877) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.

UniProt ID：

P41235

浓度：

1 mg/mL

基因名称：

HNF4A

货号：

T014|TMAB-00877