叉头蛋白P3重组兔单抗|TMAB-00711|TargetMol

中文名称：

叉头蛋白P3重组兔单抗

英文名称：

Anti-FOXP3 Antibody (7Z785)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Antibody

种属：

Human

应用：

WB

反应性：

Human

宿主：

Rabbit

靶点：

FoxP3

克隆性：

Monoclonal

免疫原：

Recombinant Protein: human FOXP3 protein, around C-terminal 150aa

亚型：

IgG

验证方法：

1. Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-FOXP3 antibody (TMAB-00711) Dilution: 2 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 2. 25 μg total protein per Lane of various lysates probed with FOXP3 monoclonal antibody, unconjugated (TMAB-00711) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 3. The MJ (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-FOXP3 antibody (TMAB-00711,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. 4. 4% Paraformaldehyde-fixed MJ (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (FOXP3) monoclonal Antibody, unconjugated (TMAB-00711) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

UniProt ID：

Q9BZS1

克隆号：

7Z785

浓度：

1 mg/mL

基因名称：

FoxP3

货号：

T014|TMAB-00711