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Peroxidase-conjugated AffiniPure® Goat Anti-Human IgG, Fcγ Fragment Specific
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上海 更新日期:2026-03-23

上海卓升科技有限公司

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产品详情:

中文名称:
Jackson抗体
英文名称:
Peroxidase-conjugated AffiniPure® Goat Anti-Human IgG, Fcγ Fragment Specific
品牌:
Jackson
产地:
美国
重组:
应用:
1
种属反应性:
2
宿主:
3
偶联物:
4
靶点:
5

美国Jackson抗体代理——上海卓升科技有限公司,更多产品,请咨询

抗原名称

IgG, Fcγ Fragment
宿主
山羊

Target: Human
Host: Goat
Antibody Format: Whole IgG
Specificity: IgG, Fcγ fragment specific
Conjugate: Horseradish Peroxidase
Product Category: Whole IgG Affinity-Purified Antibodies
Clonality: Polyclonal
RRID: AB_2337579

Information
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Usage
Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)

Suggested Working Concentration or Dilution Range:

1:500 - 1:5,000 for immunohisto/cytochemistry
1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates
1:10,000 - 1:200,000 for Western blotting with ECL substrates

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Horseradish Peroxidase



Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

应用
反应种属
Human



Jackson抗体;Jackson 109-545-003;Jackson 109-035-008;Jackson109-545-003代理;Jackson109-035-008代理;

公司简介

上海卓升科技有限公司成立于2020年,在上海拥有1500平米办公及仓储大楼,专业经营各类生物以及化学试剂。优势代理销售美国PylamDyes、英国 inspiralis、lifesciencegroup、丹麦Immudex、英国Almac、美国coriell Polysciences、Enzyme酶、日本Hatano、Ethosbiosciences、Complementtech、BD、CSTBiochain、丹麦Dako 、invitek-molecular、Evrogene、Crystal Chem、美国MEDNA、Quickzyme等几百家品牌产品,覆盖基因、蛋白、细胞、组织、血清等各种生物研究,广泛应用于生命科学研究、诊断检测、生物医药、分析检测等领域。

成立日期 (6年)
注册资本 100万
员工人数 10-50人
年营业额 ¥ 100万-300万
经营模式 工厂,试剂,定制,服务
主营行业 医药中间体,生化试剂,诊断试剂,有机合成试剂

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