PKA底物多肽CREBtide/149155-45-3/CREBtide

Product Name：CREBtide

CAS No.：149155-45-3

Synonyms：PKA底物多肽CREBtide

Catalog No.：218P594

Brand：Typeptide Biotech（肽研生物）

Description：CREBtide是一种合成的 13 个氨基酸肽，已被报道为 PKA 底物。delta-CREB 是 cAMP 反应元件结合蛋白 (CREB) 的剪接变体。一种基于 delta-CREB 磷酸化序列的合成肽。 delta-CREB 和 CREBtide 被测试为 cAMP 依赖性蛋白激酶 (cAK) 的底物。 cAK 对 CREBtide 磷酸化的表观 Km 为 3.9 μM，比最常用于 cAK 测量的合成肽底物 Kemptide (Km=39 μM) 低 10 倍。 CREBtide 的 Vmax 值为 12.4 mumol/(min.mg)，Kemptide 的 Vmax 值为 9.8 mumol/(min.mg)。 cGMP 依赖性蛋白激酶 (cGK) 对 CREBtide 磷酸化的表观 Km 为 2.9 μM，Vmax 值为 3.2 mumol/(min.mg)。 与 cAK 相比， delta-CREB 和 CREBtide 被 cGK 磷酸化的速率要慢得多，这意味着 delta-CREB 表现出的高 cAK/cGK 特异性被肽保留

CREBide is a synthetic 13 amino acid peptide that has been reported as a PKA substrate. Delta CREB is a splicing variant of cAMP response element binding protein (CREB). A synthetic peptide based on delta CREB phosphorylation sequence. Delta CREB and CREBide were tested as substrates for cAMP dependent protein kinase (cAK). The apparent Km of cAK for CREBtide phosphorylation is 3.9 μ M, which is 10 times lower than the synthetic peptide substrate Kemptide (Km=39 μ M) most commonly used for cAK measurement. The Vmax value of CREBtide is 12.4 mumol/(min. mg), and the Vmax value of Kemptide is 9.8 mumol/(min. mg). The apparent Km of cGMP dependent protein kinase (cGK) for CREBtide phosphorylation is 2.9 μ M, with a Vmax value of 3.2 mumol/(min. mg). Compared to cAK, delta CREB and CREBide are phosphorylated by cGK at a much slower rate, indicating that the high cAK/cGK specificity exhibited by delta CREB is preserved by the peptide

Purity (HPLC) ≥98.0%

Single impurity≤2%

Peptide content≥75.0%

Amino Acid Composition≤±15%

Bacterial Endotoxins≤50EU/mg

Solubility：H₂O/DMSO

Vacuum-packed with aluminum foil bag or transparent bag.Various weights can be packed according to your requirements.

Storage:Cool dry place( Store at -20°C, away from the light)

Remark:For Research Use Only. Not for human use.

订购联系方式：

南京肽研生物生物科技有限公司

手机号：19961912513(微信同号)

QQ: 2178255659

邮箱：sales@typeptide.com

联系人：顾经理

申明：本产品仅供科研使用，不得用于人体。