丝裂原活化蛋白激酶1/2单克隆抗体

丝裂原活化蛋白激酶1/2单克隆抗体

商品介绍

英文别名：P44/42 MAPK; ERK; ERK-1; ERT 2; ERT2; Extracellular Signal Regulated Kinase 1; Extracellular signal related kinase 1; Extracellular signal-regulated kinase 1; HGNC6877; HS44KDAP; HUMKER1A; Insulin Stimulated MAP2 Kinase; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP Kinase; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK; MAPK1; Mapk3; MGC20180; Microtubule Associated Protein 2 Kinase; Microtubule-associated protein 2 kinase; Mitogen Activated Protein Kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; MK03_HUMAN; OTTHUMP00000174538; OTTHUMP00000174541; p44 ERK1; p44 MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM 3; PRKM3; Protein Kinase Mitogen Activated 3.
产品应用：WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
交叉反应：Human,Mouse,Rat
抗体来源：Mouse
免疫原：KLH conjugated synthetic peptide derived from human ERK1/2
亚型;IgG

性状:Size : 50ul/100ul/200ul
Liquid

Size : 200ug (PBS only)
Lyophilized
Note: Centrifuge tubes before opening. Reconstitute the lyophilized product in distilled water. Optimal concentration should be determined by the end user.
纯化方法:affinity purified by Protein G
克隆类型:Monoclonal
克隆号:8C2

浓度:1mg/ml

储存液：Size : 50ul/100ul/200ul
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Size : 200ug (PBS only)
0.01M PBS

保存条件:Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
应用范围及推荐稀释比例

Western Blot (WB)：1:100–500

ELISA：1:500–1000

免疫组化（石蜡切片需抗原修复）：

IHC-P：1:100–500

IHC-F：1:100–500

免疫荧光 (IF)：1:100–500

其他应用：尚未测试，可根据实验需求优化稀释比例。

储存条件

储存液：0.01M TBS (pH 7.4)，含1% BSA、0.03% Proclin300及50%甘油。

保存温度：-20°C长期保存，避免反复冻融。

产品背景

丝裂原活化蛋白激酶（MAPK1/2，即ERK1/2）是细胞信号转导通路的核心组分，参与调控细胞增殖、分化、转录及发育等过程。本抗体特异性识别磷酸化ERK1/2（Thr197/Thr202位点），适用于检测相关信号通路的激活状

功能：

参与细胞信号转导，调控增殖、分化等过程。

部分产品标注可激活补体或增强巨噬细胞功能（如IgG类抗体）。

注意事项

用途限制：仅限科研使用，不可用于临床诊断或治疗。

保存与运输：需避光、避免反复冻融，长期保存建议分装。

实验优化：建议预实验确定最佳稀释比例及条件。

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