磷酸化丝裂原活化蛋白激酶1抗体

商品属性：

英文名称：phospho-Erk1 (Thr202 + Tyr204) Rabbit pAb

中文名称：磷酸化丝裂原活化蛋白激酶1抗体

英文别名：Erk1(pT202/pY204); ERK/MAPK(phospho T202/Y204); ERK1(phospho T202); p-ERK1(phospho T202); p44/42 MAP Kinase(phospho-Thr202); ERK; ERK-1; ERT 2; ERT2; Extracellular Signal Regulated Kinase 1; Extracellular signal related kinase 1; Extracellular signal-regulated kinase 1; HGNC6877; HS44KDAP; HUMKER1A; Insulin Stimulated MAP2 Kinase; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP Kinase; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK; MAPK1; Mapk3; MGC20180; Microtubule Associated Protein 2 Kinase; Microtubule-associated protein 2 kinase; Mitogen Activated Protein Kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; MK03_HUMAN; OTTHUMP00000174538; OTTHUMP00000174541; p44 ERK1; p44 MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM 3; PRKM3; Protein Kinase Mitogen Activated 3.

交叉反应：Human, Mouse, Rat (predicted: Rabbit, Cow, Chicken, Dog, GuineaPig, Horse)

抗体来源：Rabbit

免疫原：KLH conjugated Synthesised phosphopeptide derived from rat ERK1 around the phosphorylation site of Thr201/204 : FL(p-T)E(p-Y)VA

亚型：IgG

性状：Liquid

 纯化方法：affinity purified by Protein A

克隆类型：Polyclonal

理论分子量：43 kDa

浓度：1mg/ml

储存液：0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

研究领域：

Neuroscience > Neurology process > Neurodegenerative disease > Alzheimer's disease > Tangles & Tau

Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > MAPK Pathway

Stem Cells > Signaling Pathways > TGF beta > Cytoplasmic

抗体特性

宿主与克隆性：通常为兔源多克隆抗体（Polyclonal），具有较广的物种交叉反应性（人、小鼠、大鼠等）。

免疫原：以KLH偶联的、含MAPK1磷酸化位点（Thr202/Tyr204）附近序列的合成肽段为免疫原，确保抗体特异性识别磷酸化表位。

纯化方法：多采用蛋白A/G亲和层析或抗原亲和纯化，保证抗体纯度和实验可重复性。

应用范围

Western Blot (WB)：检测细胞或组织样本中MAPK1的磷酸化水平，常用稀释比例1:500-2000。

免疫组化（IHC）、免疫荧光（IF）：用于定位组织或细胞内磷酸化MAPK1的分布情况，稀释比例1:100-500。

ELISA：定量检测样本中磷酸化MAPK1的含量，稀释比例1:5000-10000。

物种交叉反应：可识别人、小鼠、大鼠、狗、猪、牛等多种物种的MAPK1蛋白。

储存与稳定性

储存条件：-20℃保存，避免反复冻融，分装推荐。

保存液：通常为TBS缓冲液（pH7.4）含1%BSA、0.03% Proclin300和50%甘油。

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