人MMP-9蛋白, His Tag

Human MMP-9 Protein, His Tag (active enzyme)

分子别名（Synonym）

MMP9, CLG4B, GELB, MANDP2, Gelatinase B

表达区间及表达系统（Source）

Human MMP-9, His Tag (MM9-H5221) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 469 (Accession # AAH06093). It needs to be activated by agents such as APMA in vitro to have hydrolytic activity.

Predicted N-terminus: Ala 20

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 50.8 kDa. The protein migrates as 58-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

基质金属肽酶9（MMP-9）又称92 kDa IV型胶原酶、92 kDa明胶酶或明胶酶B（GELB）、CLG4B，由中性粒细胞、巨噬细胞及多种转化细胞分泌，是结构域组成与活性调控机制最为复杂的家族成员。从结构上看，MMP-9可分为五个独立结构域：激活时可被切割的前结构域；由三个连续Ⅱ型纤连蛋白单元构成的明胶结合结构域；含锌离子结合位点的催化结构域；富含脯氨酸的连接区以及羧基末端的血红素结合蛋白样结构域。该酶能酶解多种底物，包括明胶、IV型和V型胶原以及弹性蛋白。MMP-9参与系统性红斑狼疮、类风湿关节炎、多发性硬化等多种自身免疫疾病的发生发展，被视为潜在的治疗靶点。