PE-Labeled人FAP-HP245蛋白, His Tag

PE-Labeled Human FAP Protein, His Tag (Site-specific conjugation)

分子别名（Synonym）

FAP,FAPalpha,SIMP,Seprase,APCE

表达区间及表达系统（Source）

PE-Labeled Human FAP, His Tag (FAP-HP245) is produced via site-specific conjugation of PE to Human FAP, His Tag under optimal conditions with a proprietary technology. Human FAP, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 93.1 kDa.

偶联（Conjugate）

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

应用说明（Application）

Please note that this product is NOT compatible to streptavidin detection system.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

成纤维细胞活化蛋白（FAP，亦称为seprase）是一种II型跨膜丝氨酸蛋白酶。其细胞膜形式与可溶形式均具有脯氨酸后切割内肽酶活性，对Ala/Ser-Gly-Pro-Ser/Asn/Ala共识序列表现出显著偏好。能降解明胶及热变性I型胶原。同时具有二肽基肽酶活性，偏好底物为Ala-Pro、Ile-Pro、Gly-Pro、Arg-Pro及Pro-Pro。

细胞膜形式的FAP通过与DPP4、PLAUR或整合素结合，参与细胞外基质的细胞周围蛋白水解过程，从而促进细胞在细胞外基质中的粘附、迁移与侵袭。通过降解蛋白聚糖短蛋白聚糖，促进胶质瘤细胞在脑实质中的侵袭。在黑色素细胞中，FAP以不依赖于丝氨酸蛋白酶活性的方式，通过调控细胞增殖与存活发挥肿瘤抑制功能。