食蟹猴TSLP蛋白, His,Avitag

Biotinylated Cynomolgus TSLP Protein, His,Avitag™ (MALS verified)

分子别名（Synonym）

TSLP

表达区间及表达系统（Source）

Biotinylated Cynomolgus TSLP, His,Avitag (TSP-C82E3) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # A0A7N9CAT7-1).

Predicted N-terminus: Tyr 29

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 18.8 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE).

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

胸腺基质淋巴细胞生成素（TSLP）是一种上皮细胞源性细胞因子，广泛表达于上皮细胞，并参与炎症性皮肤疾病的病理过程。人类TSLP cDNA编码一个由159个氨基酸残基组成的前体蛋白，其包含一段28个氨基酸的信号肽序列（4, 5）。研究证实，人类TSLP具有多种生物学功能：参与建立非缺失性中枢耐受，增强上皮诱导的类别转换，诱发特应性疾病，并维持肠道非炎症环境。在响应人类TSLP的多种细胞中，CD11c+树突状细胞是其最明确的靶细胞。