人4-1BB / TNFRSF9蛋白, Fc Tag

Human 4-1BB / TNFRSF9 Protein, Fc Tag (MALS verified)

分子别名（Synonym）

TNFRSF9,4-1BB,CD137,CDw137,ILA

表达区间及表达系统（Source）

Human 4-1BB, Fc Tag (41B-H5258) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # NP_001552.2).

Predicted N-terminus: Leu 24

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 43.4 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

4-1BB又称CD137，亦被称为肿瘤坏死因子受体超家族成员9（TNFRSF9）或淋巴细胞活化诱导分子（ILA），属于肿瘤坏死因子（TNF）受体超家族的共刺激分子。CD137可在活化的T细胞表面表达，但在CD8阳性T细胞上的表达程度显著高于CD4阳性T细胞。此外，树突状细胞、滤泡树突状细胞、自然杀伤细胞、粒细胞以及炎症部位血管壁细胞也表达CD137。CD137最明确的生物学功能是对活化T细胞的共刺激作用：其交联作用能促进T细胞增殖、增强IL-2分泌、提升细胞存活率并强化溶细胞活性。研究还表明，CD137能增强小鼠体内的免疫活性以清除肿瘤。当T细胞受体/CD3复合物被激活时，CD137可增强活化诱导的T细胞凋亡。值得注意的是，4-1BB/4-1BBL共刺激通路能增强机体对多种病毒的二次细胞毒性T淋巴细胞反应，同时提升抗肿瘤免疫力。因此，4-1BB成为人类癌症免疫治疗的重要靶点。目前已证实CD137能与TRAF2蛋白发生相互作用。