小鼠FCGR2B/CD32b蛋白, His Tag

Mouse Fc gamma RIIB / CD32b Protein, His Tag (SPR & BLI & MALS verified)

分子别名（Synonym）

FCGR

表达区间及表达系统（Source）

Mouse CD32b, His Tag (CDB-M52H7) is expressed from human 293 cells (HEK293). It contains AA Thr 40 - Arg 217 (Accession # NP_001070657.1).

Predicted N-terminus: Thr 40

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 22.2 kDa. The protein migrates as 32-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

IgG Fc段受体（FcγR）是免疫球蛋白超家族成员，在免疫反应的激活或抑制中发挥作用。目前公认人类FcγR可分为三类：RI（CD64）、RII（CD32）和RIII（CD16），这些受体可产生多种亚型。

人类FcγRII/CD32由三个基因（A、B、C）编码，而小鼠FcγRIIB（CD32B）仅有一个基因。CD32是一种低亲和力IgG受体。低亲和力免疫球蛋白γ Fc段受体II-b（FCGR2B）亦称为CD32b、FCG2、IGFR2。CD32B表达于B细胞和髓样树突细胞表面：B细胞表面的CD32B交联可下调抗体产生，并在某些情况下促进细胞凋亡；树突状细胞表面的CD32B共交联则会抑制细胞成熟并阻断细胞活化。CD32B还可能作为恶性肿瘤单克隆抗体治疗的靶点。