人EGFRvIII蛋白, His,Avitag

Biotinylated Human EGFRvIII Protein, His,Avitag™ (MALS verified)

分子别名（Synonym）

EGFRvIII

表达区间及表达系统（Source）

Biotinylated Human EGFRvIII, His,Avitag (EGR-H82E0) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 378 (Accession # NP_001333870.1).

Predicted N-terminus: Leu 25

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 42.2 kDa. The protein migrates as 60-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

表皮生长因子受体（EGFR，亦称ErbB-1，人类中称为HER1）是表皮生长因子家族胞外蛋白配体的细胞表面受体。作为ErbB受体家族成员，该亚家族包含四种密切相关的受体酪氨酸激酶：EGFR（ErbB-1）、HER2/c-neu（ErbB-2）、Her3（ErbB-3）及Her4（ErbB-4）。影响EGFR表达或活性的突变可能诱发癌症。

III型EGF缺失突变体受体（EGFRvIII）是最常见的突变形式，最初在人类原发性胶质母细胞瘤中发现。这种肿瘤特异性抗原具有不依赖配体激活的特性，其酪氨酸激酶结构域呈持续性激活状态，已被证实在多种人类恶性肿瘤中存在。近年来，EGFRvIII已被选为嵌合抗原受体T细胞研究的重要靶点。