人SCF / KITLG蛋白, Avitag,His Tag

Biotinylated Human SCF / KITLG Protein, Avitag™,His Tag

分子别名（Synonym）

KITLG,FPH2,KL-1,Kitl,MGF,SCF,SF,SHEP7,KL

表达区间及表达系统（Source）

Biotinylated Human SCF, Avitag,His Tag (SCF-H82E1) is expressed from human 293 cells (HEK293). It contains AA Glu 26 - Ala 190 (Accession # AAH69797).

Predicted N-terminus: Glu 26

蛋白结构（Molecular Characterization）

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 21.7 kDa. The protein migrates as 26-38 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍 

干细胞因子（SCF）亦称kit配体、KL、Steel因子、KITLG、FPH2、KL-1、Kitl、MGF、SF或SHEP7，是一种能与c-Kit受体（CD117）结合的细胞因子。该因子既可作为跨膜蛋白存在，也可表现为可溶性蛋白形式。这种细胞因子在造血作用（血细胞形成）、精子发生和黑色素生成过程中发挥重要功能。其可溶性及跨膜形式由同一RNA转录本通过可变剪接形成。

成纤维细胞与内皮细胞可同时产生可溶性及跨膜型SCF。可溶性SCF分子量为18.5 kDa，并以二聚体形式存在。在胚胎发育过程中，SCF对造血功能具有关键调控作用。胎儿肝脏、骨髓等造血部位均表达SCF。在发育过程中，SCF还通过调控黑色素细胞（负责产生黑色素及控制色素沉着的细胞）的定位发挥重要作用。

SCF参与调控骨髓干细胞微环境中的造血干细胞（HSCs）。在体外培养体系中，SCF可与其他细胞因子联用于造血干细胞及造血祖细胞的培养。这些细胞的体外扩增技术将推动骨髓移植领域的发展——通过移植造血干细胞至患者体内，重建其造血功能。