PE-Labeled人Siglec-3 / CD33蛋白, His Tag

PE-Labeled Human Siglec-3 / CD33 Protein, His Tag (Site-specific conjugation)

分子别名（Synonym）

CD33,SIGLEC3,gp67

表达区间及表达系统（Source）

PE-Labeled Human Siglec-3, His Tag (CD3-HP2E3) is produced via site-specific conjugation of PE to Human Siglec-3, His Tag under optimal conditions with a proprietary technology. Human Siglec-3, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).

Predicted N-terminus: Asp 18

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 29.4 kDa.

偶联（Conjugate）

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

应用说明（Application）

Evaluation of anti-CD33 CAR expression by flow cytometry. Please note that this product is NOT compatible to streptavidin detection system.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

髓系细胞表面抗原CD33亦称为SIGLEC3、Siglecs（唾液酸结合免疫球蛋白样凝集素）及GP67，是一种属于免疫球蛋白超家族和SIGLEC（唾液酸结合免疫球蛋白样凝集素）家族的单次跨膜I型膜蛋白。人源CD33/Siglec-3 cDNA编码的364个氨基酸多肽链包含一个疏水信号肽、一个N端免疫球蛋白样V型结构域、一个免疫球蛋白样C2型结构域、一个跨膜区及一个胞质尾区。CD33/Siglec-3通常被认为是髓系特异性标志物，但也在部分淋巴样细胞中表达。在免疫应答过程中，CD33/Siglec-3可通过其SH2结构域招募胞质磷酸酶，使信号分子去磷酸化从而阻断信号传导，经配体诱导的酪氨酸磷酸化后可作为抑制性受体发挥作用。CD33/Siglec-3能够诱导急性髓系白血病细胞发生凋亡。