人IL3RA / CD123蛋白, His Tag

Human IL-3 R alpha / CD123 Protein, His Tag (MALS & SPR verified)

分子别名（Synonym）

IL3R,IL3RA,IL-3Ra,IL-3R-alpha,IL3RAY,IL3RX,IL3RY,CD123 antigen,CD123,hIL3Ra,hIL-3Ra,MGC34174,IL-3 R alpha

表达区间及表达系统（Source）

Human IL-3 R alpha, His Tag (ILA-H52H6) is expressed from human 293 cells (HEK293). It contains AA Thr 19 - Arg 305  (Accession # P26951-1).

Predicted N-terminus: Thr 19

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 35.0 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

白细胞介素3受体α链（低亲和力）（IL3RA）亦称为分化簇123（CD123），是造血素受体超家族中一种70kD的糖蛋白。该蛋白通过与IL-5及粒细胞-巨噬细胞集落刺激因子（GM-CSF）受体共有的β亚基结合，形成IL-3的高亲和力受体。由于在急性髓系白血病（AML）中白细胞介素-3受体α链（CD123）的表达水平显著高于正常造血干细胞，该蛋白已被视为潜在的免疫治疗靶点。