小鼠TSLP蛋白, His Tag(MALS verified)

Mouse TSLP Protein, His Tag (MALS verified)

分子别名（Synonym）

TSLP

表达区间及表达系统（Source）

Mouse TSLP, His Tag (TSP-M52H8) is expressed from human 293 cells (HEK293). It contains AA Tyr 20 - Glu 140 (Accession # Q9JIE6-1).

Predicted N-terminus: Tyr 20

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 15.9 kDa. The protein migrates as 23-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

胸腺基质淋巴生成素（TSLP）是一种上皮细胞源性细胞因子，广泛表达于上皮细胞并参与炎症性皮肤病的病理过程。人类TSLP cDNA编码由159个氨基酸残基组成的前体蛋白，其中包含28个氨基酸信号肽序列（4,5）。研究表明，人类TSLP具有促进非缺失性中枢耐受、增强上皮诱导的类别转换、诱发特应性疾病以及维持肠道非炎症环境等功能。在响应人类TSLP的多种细胞中，CD11c+树突状细胞是特征最为明确的靶细胞。