人HGF蛋白, His Tag(MALS verified)

Human HGF Protein, His Tag (MALS verified)

分子别名（Synonym）

HGF, HPTA, SF

表达区间及表达系统（Source）

Human HGF Protein, His Tag (HGF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Ser 728 (Accession # P14210-1).

Predicted N-terminus: Gln 32

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 81.6 kDa (α chain 53.7 kDa and β chain 27.9 kDa). The protein migrates as 80-90 kDa (alpha & Beta chain), 58-63 kDa (alpha chain), 33 kDa (Beta chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method / rFC method.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative

纯度（Purity）

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

肝细胞生长因子（HGF）是一种旁分泌型细胞生长、运动与形态发生因子。其通过结合并促进酪氨酸激酶受体MET的二聚化，成为该受体的激活配体。该因子由间充质细胞分泌，作为多功能细胞因子主要作用于上皮源性的细胞。其刺激有丝分裂、细胞运动及基质侵袭的能力，使其在血管生成、肿瘤发生和组织再生中发挥核心作用。此外，HGF已被发现与多种癌症的发生发展密切相关，包括肺、胰腺、甲状腺、结肠及乳腺癌。