人IL-4蛋白, Fc Tag (MALS verified)

Human IL-4 Protein, Fc Tag (MALS verified)

分子别名（Synonym）

IL4,BCGF1,BSF1

表达区间及表达系统（Source）

Human IL-4 Protein, Fc Tag (IL4-H5253) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P05112-1).

Predicted N-terminus: His 25

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 41.4 kDa. The protein migrates as 46-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

白细胞介素4（IL-4）是一种可诱导初始辅助T细胞（从Th0细胞向Th2细胞）分化的细胞因子。在Th2型免疫应答（尤其见于过敏反应与寄生虫感染过程中）产生的IL-4和IL-13共同作用下，巨噬细胞将发生差异性活化。该细胞因子是白细胞介素4受体的配体，而该受体亦能与IL-13结合，这可能是IL-4与IL-13功能存在诸多重叠的原因之一。STAT6（信号转导与转录激活因子）已被证实在介导该细胞因子的免疫调节信号中起核心作用。最新研究发现，在Itk、KLF2、CBP及Id3等多种基因缺陷型小鼠中，IL-4在胸腺内源性CD8+ T细胞的发育过程中起着关键作用，且该过程无需抗原预先刺激。