小鼠FAP蛋白, His,Avitag

Biotinylated Mouse FAP Protein, His,Avitag™, active dimer (MALS verified)

分子别名（Synonym）

FAP,FAPalpha,SIMP,Seprase,APCE

表达区间及表达系统（Source）

Biotinylated Mouse FAP, His,Avitag (FAP-M82Q8) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 761 | Leu 26 - Asp 761 (Accession # P97321).

Predicted N-terminus: His | His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 92.7 kDa | 92.6 kDa. The protein migrates as 95-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

成纤维细胞活化蛋白（FAP，亦称为seprase）是一种II型跨膜丝氨酸蛋白酶。其膜结合形式与可溶形式均具有脯氨酸后切内肽酶水解活性，尤其偏好识别Ala/Ser-Gly-Pro-Ser/Asn/Ala共识序列。该酶还能降解明胶及热变性I型胶原蛋白，同时具备二肽基肽酶活性，可特异性水解Ala-Pro、Ile-Pro、Gly-Pro、Arg-Pro及Pro-Pro等二肽底物。膜结合型FAP通过与DPP4、PLAUR或整合素等分子协同作用，参与细胞外基质的胞周蛋白水解过程，进而促进细胞在基质中的黏附、迁移与侵袭。在脑实质中，可通过降解蛋白聚糖短蛋白聚糖促进胶质瘤细胞的侵袭。在黑色素细胞中，FAP以不依赖丝氨酸蛋白酶活性的方式调控细胞增殖与存活，发挥肿瘤抑制因子功能。