Biotinylated人CA125 / MUC16蛋白, Fc,Avitag

Biotinylated Human CA125 / MUC16 (13810-14451) Protein, Fc,Avitag™

分子别名（Synonym）

CA125,CA-125,CA125MUC-16,FLJ14303,MUC16,mucin 16,mucin-16

表达区间及表达系统（Source）

Biotinylated Human CA125 (13810-14451), Fc,Avitag (CA5-H82F8) is expressed from human 293 cells (HEK293). It contains AA Pro 13810 - Pro 14451 (Accession # Q8WXI7-1).

Predicted N-terminus: Pro 13810

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 100.0 kDa. The protein migrates as 120-160 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

CA125（亦称MUC16）是一种黏蛋白，以I型跨膜蛋白或分泌型蛋白形式存在，可用于监测上皮性卵巢癌的治疗进程。该分子本质上是一种以O-连接为主的糖蛋白，由于在体液存续期间侧链持续发生去糖基化作用，其大小和电荷均呈现异质性。在自然条件下，CA125以超大分子复合物形式存在（分子量可达400万道尔顿）。