Biotinylated整合素Integrin αvβ8蛋白, His,Avitag&Tag Free

Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

分子别名（Synonym）

Integrin alpha V beta 8,ITGAV&ITGB8

表达区间及表达系统（Source）

Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His,Avitag&Tag Free (IT8-H82W5) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8) (Accession # NP_002201.1 (ITGAV) & NP_002205.1 (ITGB8)).

Predicted N-terminus: Phe 31 (ITGAV) & Glu 43 (ITGB8)

蛋白结构（Molecular Characterization）

Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 114.7 kDa (ITGAV) and 76.5 kDa (ITGB8). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 135 kDa (ITGAV) and 80 kDa (ITGB8) respectively due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

整合素αVβ8（ITGAV与ITGB8或ITGAVB8）表达于卵黄囊、胎盘、脑组织中的血管周围星形胶质细胞、施万细胞、肾小球系膜细胞及肺上皮细胞。与其他αV整合素不同，ITGAVB8似乎不呈现不同的激活状态，其胞质尾部不与细胞骨架连接。该整合素可结合含RGD基序的配体，包括玻连蛋白、纤维蛋白以及潜伏TGF-β复合物中的潜伏相关肽（LAP）。αVβ8与LAP的高亲和力结合允许MT1-MMP对其进行蛋白水解切割，从而释放活性TGF-β。此作用机制不同于另一种αV整合素——αVβ6，后者通过非蛋白水解机制从潜伏状态激活TGF-β。TGF-β激活的下游效应包括调控细胞生长及相关血管化过程。