食蟹猴HGF R / c-MET蛋白, His Tag

Cynomolgus HGF R / c-MET Protein, His Tag (MALS verified)

分子别名（Synonym）

MET, AUTS9, HGFR, RCCP2, c-Met

表达区间及表达系统（Source）

Cynomolgus HGF R, His Tag (MET-C52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Asn 930 (Accession # A0A2K5UM36-1).

Predicted N-terminus: Glu 25

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 103.2 kDa. The protein migrates as 80 kDa under reducing (R) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

肝细胞生长因子受体（HGFR）又称间充质-上皮转化因子（MET）、c-Met，是一种糖基化受体酪氨酸激酶，在上皮形态发生和癌症发展中发挥核心作用。HGFR蛋白具有酪氨酸激酶活性。其初级单链前体蛋白经翻译后剪切产生α和β亚基，通过二硫键连接形成成熟受体。HGFR通常表达于上皮来源细胞，而HGF的表达仅限于间充质来源细胞。在HGF刺激下，HGFR可诱导多种生物反应，共同引发名为"侵袭性生长"的生物学程序。癌症中HGFR的异常活化与不良预后相关，其异常激活会引发肿瘤生长、形成供给营养的新生血管（血管生成）及癌症向其他器官扩散（转移）。HGFR在多种人类恶性肿瘤中均出现失调，包括肾、肝、胃、乳腺及脑部肿瘤。正常情况下仅干细胞和祖细胞表达HGFR，而癌症干细胞被认为劫持了正常干细胞表达HGFR的能力，从而成为癌症持续存在并向体内其他部位扩散的根源。HGFR基因的多种突变与乳头状肾细胞癌相关。该受体介导的"侵袭性生长"程序可触发有丝分裂和形态发生。