食蟹猴PD-1 / PDCD1蛋白, His Tag

Cynomolgus PD-1 / PDCD1 Protein, His Tag (MALS verified)

分子别名（Synonym）

PDCD1,PD1,CD279,SLEB2

表达区间及表达系统（Source）

Cynomolgus PD-1, His Tag (PD1-C52H5) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # B0LAJ3).

Predicted N-terminus: Leu 25

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 17.8 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

程序性死亡蛋白1（PD-1）亦称CD279和PDCD1，是一种I型膜蛋白，属于延伸的CD28/CTLA-4 T细胞调节家族成员。PDCD1表达于活化的T细胞、B细胞、巨噬细胞、髓系细胞及部分胸腺细胞表面。PD-1拥有两个B7家族配体PD-L1与PD-L2。经IFN-γ处理后，PD-L1几乎在所有小鼠肿瘤细胞系（包括PA1骨髓瘤、P815肥大细胞瘤及B16黑色素瘤）中均有表达；而PD-L2表达范围更为局限，主要存在于树突状细胞及少数肿瘤细胞系。PD-1通过抑制PI3K/AKT通路的激活与转导，进而抑制T细胞增殖及IL-1、IL-4、IL-10和IFN-γ等相关细胞因子的产生。此外，PD-1共交联可通过去磷酸化关键信号转导分子来抑制BCR介导的信号。体外实验中，用PD-L1-Ig处理抗CD3刺激的T细胞可导致T细胞增殖与IFN-γ分泌减少。目前正在研发靶向PD-1的单克隆抗体，通过增强免疫系统功能用于癌症治疗。