食蟹猴APRIL / TNFSF13蛋白, His, Flag Tag

Cynomolgus APRIL / TNFSF13 Protein, His,Flag Tag, active trimer (MALS verified)

分子别名（Synonym）

APRIL, TNFSF13, TALL-2, TRDL-1, CD256, TALL2, ZTNF2

表达区间及表达系统（Source）

Cynomolgus APRIL, His,Flag Tag (APL-C52D2) is expressed from human 293 cells (HEK293). It contains AA Gln 111 - Leu 250 (Accession # XP_005582818.1) trimer Design.

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

The protein has a calculated MW of 50.4 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

增殖诱导配体（APRIL）亦称肿瘤坏死因子配体超家族成员13、TALL-2、TRDL-1、CD256或TNFSF13，是一种能够与TNFRSF13B/TACI及TNFRSF17/BCMA受体结合的细胞因子。作为肿瘤坏死因子家族成员，APRIL主要与血液系统恶性肿瘤相关。

与其功能密切相关的TNF家族配体——B细胞活化因子（BAFF）与增殖诱导配体（APRIL）共同参与成熟B淋巴细胞的生成与维持。BAFF和APRIL均可组装成同源三聚体，通过结合并激活多种部分共用的受体发挥作用。不同化学计量组成的BAFF-APRIL异源多聚体具有独特的受体结合特性与生物学活性。此外，研究发现在GC细胞中，APRIL的表达受到miR-145的调控。