Biotinylated人CD5蛋白, premium grade

Biotinylated Human CD5 Protein, His,Avitag™, premium grade

分子别名（Synonym）

CD5,LEU1

表达区间及表达系统（Source）

Biotinylated Human CD5, His,Avitag, premium grade (CD5-H82E5) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Pro 372 (Accession # P06127-1).

Predicted N-terminus: Arg 25

该产品在严格的质量控制体系下生产，执行包括无菌检测和内毒素检测在内的全面测试方案。产品性能经过严谨验证，并针对细胞培养用途及临床前研究阶段的其他应用进行兼容性测试。

GMP-CD5H24是CD5-H82E5蛋白的GMP级别版本。两种版本蛋白质的性能参数完全一致，可确保终端用户从早期临床前研究到后续临床阶段实现无缝衔接的技术过渡。

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 42.2 kDa. The protein migrates as 52 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotinylation）

As determined by Quantitative ELISA binding assay against streptavidin.

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method / rFC method.

宿主蛋白残留（Host Cell Protein）

<0.5 ng/µg of protein tested by ELISA.

宿主核酸残留（Host Cell DNA）

<0.02 ng/μg of protein tested by qPCR.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

T细胞表面糖蛋白CD5（亦称淋巴细胞抗原T1/Leu-1及LEU1）的酪氨酸残基可被LYN激酶磷酸化，从而为PTPN6/SHP-1形成结合位点。CD5可能作为受体参与调节T细胞增殖，在人类B细胞的不同发育阶段和活化状态中均有表达。

作为T细胞受体（TCR）与B细胞受体（BCR）信号通路的明确负向调控因子，CD5阳性细胞还能通过分泌IL-10等细胞因子来阻止自身免疫现象的产生。不同B细胞与T细胞亚群的发育、筛选及功能调控，或其优势存活状态，可能直接或间接依赖于CD5或类CD5分子所关联的特定聚糖结构。