Biotinylated人IL-17F蛋白, His, Avitag

Biotinylated Human IL-17F Protein, His,Avitag™ (MALS verified)

分子别名（Synonym）

IL-17F, Interleukin-17F, Cytokine ML-1

表达区间及表达系统（Source）

Biotinylated Human IL-17F, His,Avitag (ILF-H82Q9) is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # Q96PD4-1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 18.5 kDa. The protein migrates as 25-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a dimer.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

白细胞介素17F（IL-17F）亦称白细胞介素24（IL-24）、细胞因子ML-1，是一种分泌型二硫键连接的同源二聚体，属于IL-17家族成员。人类和小鼠的IL-17家族至少包含六个成员：IL-17A、IL-17B、IL-17C、IL-17D、IL-17E（又称IL-25）及IL-17F（又称IL-24）。该家族是由结构相关细胞因子组成的蛋白群体，它们共同具有高度保守的C端区域，但N端区域及其独特生物学功能各不相同。IL-17F/IL-24在活化的CD4+T细胞及活化单核细胞中表达（静息态细胞不表达），能刺激IL-6、IL-8和粒细胞集落刺激因子等其他细胞因子的产生，并可调节软骨基质代谢。IL-17F/IL-24能促进外周血单个核细胞（PBMC）与T细胞增殖，同时具有抑制血管生成的作用。IL-17F的缺陷是导致6型家族性念珠菌病（CANDF6）的致病原因。