人IL-12B&IL-12A蛋白, His Tag&Flag Tag

Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (MALS verified)

分子别名（Synonym）

IL12, p70, Interleukin-12

表达区间及表达系统（Source）

Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (IL2-H4210) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (IL-12B) & Arg 23 - Ser 219 (IL-12A) (Accession # P29460-1 (IL12B) & P29459-1 (IL12A)).

Predicted N-terminus: Ile 23 (IL12B) & Arg 23 (IL12A)

蛋白结构（Molecular Characterization）

Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of IL-12B and IL-12A, has a calculated MW of 36.6 kDa (IL-12B) and 23.8 kDa (IL-12A). Subunit IL-12B is fused with polyhistidine tag at the C-terminus and subunit IL-12A is fused with flag tag at the C-terminus. The protein migrates as 30-38 kDa (IL12B) and 40-50 kDa (IL12A) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

白细胞介素12（IL-12）又称p70，是一种由树突状细胞、巨噬细胞和人B淋巴母细胞（NC-37）在抗原刺激下自然产生的白细胞介素。IL-12是一种异二聚体细胞因子，由IL-12A（p35）和IL-12B（p40）两个亚基组成。该细胞因子参与初始T细胞向Th1细胞的分化过程，被称为T细胞刺激因子，能促进T细胞生长并增强其功能。它可刺激T细胞和自然杀伤（NK）细胞产生γ-干扰素（IFN-γ）和肿瘤坏死因子α（TNF-α），并能降低IL-4对IFN-γ的抑制作用。IL-12在自然杀伤细胞和T淋巴细胞的生理活动中具有重要作用，同时具备抗血管生成活性，即能够阻断新生血管的形成。