人VEGFR1 / Flt-1蛋白, His Tag

Human VEGF R1 / Flt-1 Protein, His Tag (MALS verified)

分子别名（Synonym）

FLT, VEGFR1, FLT1

表达区间及表达系统（Source）

Human VEGF R1 Protein, His Tag (VE1-H52H9) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Asn 756 (Accession # P17948-1).

Predicted N-terminus: Ser 27

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 84.1 kDa. The protein migrates as 110-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

血管内皮生长因子受体1（VEGFR1）亦称Fms样酪氨酸激酶1（FLT-1）、酪氨酸蛋白激酶受体FLT，是一种属于蛋白激酶超家族、Tyr蛋白激酶家族及CSF-1/PDGF受体亚家族的I型单次跨膜蛋白兼分泌蛋白。该受体在正常肺组织及胎盘、肝、肾、心、脑组织中均有检测，特异性表达于大多数血管内皮细胞，并存在于外周血单核细胞。VEGFR1作为VEGFA、VEGFB与PGF的细胞表面受体，在胚胎血管系统发育、血管生成调控、细胞存活、细胞迁移、巨噬细胞功能、趋化作用及癌细胞侵袭中发挥关键作用。该受体可能通过抑制内皮细胞过度增殖，成为胚胎血管生成的负向调控因子。在成年期，VEGFR1可促进内皮细胞增殖、存活及血管生成。