人DLL1 / Delta1蛋白, His Tag, premium grade

Human DLL1 / Delta1 Protein, His Tag, premium grade

分子别名（Synonym）

DLL1,Delta1,H-Delta-1

表达区间及表达系统（Source）

Human DLL1 Protein, His Tag, premium grade (DL1-H52H8) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Gly 540 (Accession # NP_005609).

Predicted N-terminus: Gln 18

该产品在我们严格的质量控制体系下生产，实施包括无菌检测和内毒素检测在内的全面测试方案。产品性能经过严谨验证，确保其适用于细胞培养及早期临床前阶段的各类应用。当研发进程推进至后期临床阶段时，我们还可提供定制化的GMP蛋白质服务。我们将根据您的需求，共同开发符合细胞治疗产品生产用原材料与辅料要求的GMP级别产品。

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 58.4 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method / rFC method.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

δ样蛋白1（DLL1）亦称果蝇Delta同源蛋白1（Delta1或H-Delta-1），其结构包含一个DSL结构域及八个EGF样结构域。该蛋白可被MIB（MIB1或MIB2）泛素化，进而引发内吞作用并最终降解。在表达分布方面，DLL1在心脏和胰腺中高表达，脑组织和肌肉中表达水平较低，而在胎盘、肺、肝及肾脏中几乎不表达。此外，DLL1作为Notch受体的配体发挥作用，既能阻断祖细胞向B细胞谱系分化，又能促进具有T细胞/NK细胞前体特征的细胞群出现。